![Figure 1. Genotypic and phenotypic characterization of PU/ER(T) mice. (A) Schematic illustration of the PU/ER(T) targeting vector map that includes the vector, the PU.1 locus, and the targeted PU.1 mutant allele generated by homologous recombination. The coding sequence for the PU/ER(T) transgene is knocked into the WT PU.1 loci (exon 1 harboring the nuclear localization signal sequence) as illustrated. Double-headed arrow corresponds to the sequence used as a probe for Southern hybridization that encompasses a part of exon 1 its 5′ sequence. The restriction sites are indicated as E1, EcoRI; H3, HindIII. (B) Southern blot analysis of WT, homozygote [PU/ER(T)+/+], and heterozygote [PU/ER(T)+/−] fetal liver genomic DNA that designates the location of the 8.3-kb WT, 8.3- and 5.60-kb PU/ER(T)+/−, and 5.6-kb PU/ER(T)+/+ bands. (C) Flow cytometry analysis of day 14 ± 1 FLC suspension from PU/ER(T)+/− mice. Numbers in the bottom right quadrates indicate percentage of cells carrying the CD 11b surface antigen. Data are representative of 3 individual measurements.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/118/19/10.1182_blood-2011-03-341123/4/zh89991181610001.jpeg?Expires=1735504821&Signature=dsDCum-ram8Q0JcfLK-Ygl7BG7cruq~huVoEjaFxl4HBcsrXtzQqqHk-FpFQwFR0yrRDuElymoeGw6DbbNBGitEpHVbOT34ZWxbEs3Md0ph9ZXX1qlq0i0XbXxk37lDgYQzI7Px-304tzHeKcMwnMqpD6qMRK4RK39~nUgmazQ6HiEj73zbWOywG2RyZQbXk6jNYLqcQQtRB~X156FHR2bXE20TjncC3qYQwYYjSB9WMHy-9cUBNzPmLsJg1mhqcmYQ4OYH~IfBBDQGvb~nAddJQzXCs~GBf4saOvNuQ65V6UQy~16GLoiy2RunfFyu4zJJ2W8x0gPs5fvoYXNZ7ng__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genotypic and phenotypic characterization of PU/ER(T) mice. (A) Schematic illustration of the PU/ER(T) targeting vector map that includes the vector, the PU.1 locus, and the targeted PU.1 mutant allele generated by homologous recombination. The coding sequence for the PU/ER(T) transgene is knocked into the WT PU.1 loci (exon 1 harboring the nuclear localization signal sequence) as illustrated. Double-headed arrow corresponds to the sequence used as a probe for Southern hybridization that encompasses a part of exon 1 its 5′ sequence. The restriction sites are indicated as E1, EcoRI; H3, HindIII. (B) Southern blot analysis of WT, homozygote [PU/ER(T)+/+], and heterozygote [PU/ER(T)+/−] fetal liver genomic DNA that designates the location of the 8.3-kb WT, 8.3- and 5.60-kb PU/ER(T)+/−, and 5.6-kb PU/ER(T)+/+ bands. (C) Flow cytometry analysis of day 14 ± 1 FLC suspension from PU/ER(T)+/− mice. Numbers in the bottom right quadrates indicate percentage of cells carrying the CD 11b surface antigen. Data are representative of 3 individual measurements.
