Immunophenotype of PU/ER(T)^+/− BMDMs. (A) BMDMs from PU/ER(T)^+/− mice were treated with 4-OHT for 24 hours, and then the expression of CD 11b, F4/80, and CD 206 was measured by FACS analysis. Data are representative of 3 individual measurements. (B) PU/ER(T)^+/− BMDM cells were treated with and without 4-OHT for 24 hours and challenged with 100 ng/mL LPS. Total cellular RNA was isolated and reverse transcribed. Relative expression levels of COX-2 and iNOS were analyzed by qRT-PCR. Data are representative of the average of 3 individual measurements. (C) PU/ER(T)^+/− BMDMs were treated with and without 4-OHT and challenged with 100 ng/mL LPS. The culture supernatant was analyzed for IL-6 and TNF-α by multiplex ELISA. Data represent mean ± SEM of 4 individual measurements (*P < .01 compared with ethanol-treated cells, **P < .001 compared with PU/ER(T)^+/− LPS treatment).