High expression of CD147 correlates with high levels of FoxP3 and CD45RO and low levels of CD127 expression. (A-C) CD4⁺ T cells from human buffy coats were analyzed for their FoxP3 expression with respect to CD147. Total CD4 (A-B), CD4⁺CD25 enriched or CD4⁺CD25 depleted (C) T-cell fractions were analyzed by FACS using anti-CD4, anti-CD25, anti-CD147, and anti-FoxP3 under nonstimulating conditions. The ratio of CD4⁺CD25⁺CD147⁺ versus CD4⁺CD25⁺CD147⁻ T cells in CD4 enriched cell fractions from 13 healthy individual blood donors is shown (B). Distribution of the obtained ratios of CD4⁺CD25⁺CD147⁺ versus CD4⁺CD25⁺CD147⁻ T cells in n = 13 donors with median (line), 25-75 (box), and 2.5-97.5 (bar) percentiles shown. (D) CD147 expression in 3 subsets of CD4⁺ FoxP3⁺ T cells as defined by CD25 expression. (E) Phenotypical analysis of CD4⁺CD147⁻FoxP3⁺ and CD4⁺CD147⁺FoxP3⁺ T cells. Cell surface marker staining was determined by flow cytometry. Similar results were observed in at least 3 independent experiments for each marker, and the results of a representative experiment are shown. (F) Flow cytometric analysis using anti-CD4, anti-CD25, anti-CD147, and anti-CD127 demonstrates the negative correlation between CD147 and CD127.