American Society of Hematology

Figure 4

$Figure 4. Suppressive activity of CD147+ and CD147− Treg cells. (A) CD4+ T cells were FACS sorted into 4 major different subsets (Fraction I-IV) based on their CD147 and CD25 expression levels. (B) Responder cells, Fraction I and IV, were sorted as one fraction and labeled with CFSE. FACS sorted CD147+ Treg (Fraction II) or CD147− Treg (Fraction III) were added as indicated at ratios versus responder cells that varied from 1:1 to 1:4. Cultures were activated using anti-CD3/anti-CD2/anti-CD28-coated beads at a 1:5 ratio of beads to cells and number of proliferating cells identified in FlowJo Version 8.8.2. (C) Responder cells, Fraction I and IV were FACS sorted, CFSE labeled and cultured separately. Treg suppression assay was performed as in panel B with a CD147+ Treg (Fraction II) or CD147− Treg (Fraction III) cell (addback cells) versus responder cell ratio of 1:2. (D) Proliferation data from Treg suppression assays were normalized and the suppressive activity of CD147+ Tregs and CD147− Tregs were compared from 11 healthy individual blood donors. Statistical significance was determined with Student t test with P < .001. Median (line), 25-75 (box) and 2.5-97.5 (bar) percentiles are shown.$

Suppressive activity of CD147⁺ and CD147⁻ Treg cells. (A) CD4⁺ T cells were FACS sorted into 4 major different subsets (Fraction I-IV) based on their CD147 and CD25 expression levels. (B) Responder cells, Fraction I and IV, were sorted as one fraction and labeled with CFSE. FACS sorted CD147⁺ Treg (Fraction II) or CD147⁻ Treg (Fraction III) were added as indicated at ratios versus responder cells that varied from 1:1 to 1:4. Cultures were activated using anti-CD3/anti-CD2/anti-CD28-coated beads at a 1:5 ratio of beads to cells and number of proliferating cells identified in FlowJo Version 8.8.2. (C) Responder cells, Fraction I and IV were FACS sorted, CFSE labeled and cultured separately. Treg suppression assay was performed as in panel B with a CD147⁺ Treg (Fraction II) or CD147⁻ Treg (Fraction III) cell (addback cells) versus responder cell ratio of 1:2. (D) Proliferation data from Treg suppression assays were normalized and the suppressive activity of CD147⁺ Tregs and CD147⁻ Tregs were compared from 11 healthy individual blood donors. Statistical significance was determined with Student t test with P < .001. Median (line), 25-75 (box) and 2.5-97.5 (bar) percentiles are shown.

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