CD147 expression directly correlates with CD45RA/FoxP3-defined subsets. (A) CD4⁺ T cells were stained with an antibody cocktail consisting of conjugated anti-CD4, anti-CD147, anti-CD45RA, and anti-FoxP3 before flow cytometric analysis. The total CD4⁺ population was split into 3 different subsets depending on their CD147 expression levels, that is, CD147⁻/CD147⁺/CD147⁺⁺ and analyzed with respect to their CD45RA and FoxP3 staining. The presented figure was obtained from one representative of 6 healthy blood donors. (B) A histogram overlay analysis revealed the positive correlation of CD147 expression and the activation status of Tregs. (C) A graphic presentation of the variations observed in the 6 individuals analyzed in panel A.