Expression of CD39 and CD73 in a cohort of CLL patients. PBMCs purified from CLL patients were stained with anti–CD19-FITC and anti–CD39-PE or anti–CD73-PE. (A) Representative density plots from 2 different samples. Cumulative data on the percentage of double-positive cells or the MFI are reported in panels B and C, respectively. n = 35. (D) RT-PCR data on the mRNA levels of CD39 and CD73 in the same cohort of patients. n = 35. Mean values of expression are represented as a horizontal line. (E-F) Correlation between the expression of CD73 and CD38 (n = 299) and CD73 and ZAP-70 (n = 295), adopting the arbitrary cut-off value of 20%. (G) Percentage of CD19⁺/Ki-67⁺ cells in a subset of patients characterized by low (< 10%) and high (> 70%) CD73 expression. Data in panels E to G are expressed as box plot, where the top and the bottom of the box represent the first and the third quartiles, respectively; the horizontal line within the box indicates the median; and the whiskers represent the mean ± SEM. Statistical analysis was performed using Student t and Mann-Whitney U tests.