ADO inhibits CLL chemotaxis in response to CXCL12. (A) A conventional chemotaxis assay with Boyden chamber was adopted, using CXCL12 (60 ng/10⁶ cells/well) as chemoattractant, in the presence or absence of ADO (50μM) for the whole length of the assay (4 hours). Where indicated, cells were pretreated with CGS21680 (10μM, for 30 minutes at 37°C) or APCP (10μM, for 60 minutes at 37°C). Data are presented as fold increase or decrease, considering 1 the MI of the cells in the presence of CXCL12 alone. n = 8. (B-C) MFI and percentage of expression of CXCR4 in a cohort of 40 patients (20 CD73⁺ and 20 CD73⁻ on the basis of the 30% functional cut-off). Data are expressed as box plot, where the top and the bottom of the box represent the first and the third quartiles, respectively; the horizontal line within the box indicates the median; and the whiskers represent the mean ± SEM. Statistical analysis was performed using the Mann-Whitney U test.