Progeny of TS1 T_EM produce less IFN-γ than progeny of TS1 T_N. HA104 mice received 750cGy and 8 × 10⁶ RAG^−/− BM cells in combination with 3000 TS1 T_N, T_CM or T_EM. (A) Expression of CD62L and CD44 for CD4⁺ TS1⁺ cells in the spleen on days 7, 14 and 21. (B-E) HA104 recipients were transplanted with 3000 TS1 T_N or T_EM as described in panel A. On day 21, spleen and colon cells were isolated, stimulated with PMA and ionomycin and stained for IFN-γ and IL-2. Representative FACS plots with and without stimulation are shown for the colon (B) and for the spleen (D). The percentages of TS1 cells that are IFN-γ⁺ or IL-2⁺ and the MFIs of the IFN-γ⁺ or IL-2⁺ cells are shown for the colon (C) and the spleen (E). Data are combined from 2 independent experiments (total of 8 mice per group).