FlnA potentiates PACSIN2 F-BAR domain membrane tubulation in vitro. (A) PC/PS liposomes were incubated with GST-PACSIN2 F-BAR domain WT or P180A in the presence or absence of His-FlnA constructs, as indicated. Membrane tubulation capacity was assessed in the electron microscope. (B) Diameter of membrane tubules generated by GST-PACSIN2 (P2) F-BAR domain in the presence or absence of His-FlnA. (C) PC/PI liposomes were incubated with GST-PACSIN2 (P2) F-BAR domain and/or EGFP-FlnA and observed by light and fluorescence microscopy.