Impaired Akt phosphorylation in response to thrombin stimulation in FOG1 ki/ki platelets. WT or FOG1 ki/ki platelets were stimulated for 10 minutes with thrombin (1 U/mL), ADP (10μM), convulxin (CVX; 6nM), or the thromboxane A2 analog U46619 (TXA2; 10μM), and then cell lysates were analyzed by Western blot analysis for Akt phosphorylated at Ser⁴⁷³ (P-Akt), total Akt, or β-actin as a control. Shown is a representative Western blot analyzing an equal number of WT and FOG1 ki/ki platelets from littermate mice before or after stimulation by the indicated agonists.