Wnt/β-catenin signaling reduces ICAM-1/VCAM-1–mediated adhesion of alveolar macrophages and neutrophils to AECs. E10 cells were incubated with 50% Con_CM or Wnt3a_CM together with 200 μM Dkk1 for 12 hours and then stimulated with 20 ng/mL TNFα for 24 hours, or E10 cells were incubated with control IgG or blocking mAbs against ICAM-1 or VCAM-1 for 30 minutes and then stimulated with 20 ng/mL TNFα for 24 hours. Next, adhesion of freshly isolated AMϕ or neutrophils to E10 cells was assayed. The values are presented as a percentage of attached AMϕ or neutrophils from 3 independent experiments and are tested for statistical significance by ANOVA analysis followed by the post hoc Tukey test. Data are expressed as the mean ± SEM. *, **, ***, #, ##, and ### represent P < .05.