Clinical, cytologic, immunohistochemical, and molecular findings in VRL. (A) Representative fundus photographs of 3 VRL patients: VRL with retinal and choroidal infiltration (left), with massive infiltration at rear pole (middle) and with vitreous infiltration only (right). (B) Upper left: PVRL, 49-year-old woman, MYD88^L265P. Highly cellular vitreous body aspirate with numerous atypical lymphocytes (May-Grünwald-Giemsa staining, left). Virtually all cells stain for CD20 (immunoperoxidase, right). Lower left: PVRL, 63-year-old woman, MYD88^L265P. The aspirate shows rare atypical cells with basophilic cytoplasm (arrowheads, May-Grünwald-Giemsa staining). Clonality analysis was noncontributory and the case was considered as nondiagnostic for PVRL. Lower right: PVRL, 70-year-old woman. MYD88^L265P, monoclonal IGH rearrangement. CD20 stain. All images taken at original magnification ×400. (C) Representative example of MYD88 codon 265 locked nucleic acid (LNA)-clamped, allele-specific PCR and melting curve analysis. One case with final diagnosis uveitis shows the WT curve with the melting peak at 62.7°C, whereas case 6B, which initially was considered reactive, turned out to be MYD88^L265P-mutated, melting at 53.1°C.