MiR-155 deficiency leads to downregulation of P2ry12, P2ry2, and P2rx7, and diminished migratory ability. (A) Heat map showing the expression of purinergic receptor mRNA in a microarray analysis of WT and miR-155^−/− C57BL/6 DCs after stimulation with 50 ng/mL LPS and 5 mM ATP for 15 minutes. (B) Migration of BM-derived DCs from WT and P2ry12^−/− mice up an ATP gradient. Data were acquired from 3 independent experiments. (C) Migration of BM-derived DCs from WT and P2ry2^−/− mice up an ATP gradient. Data were acquired from 4 independent experiments. (D) Migration of BM-derived DCs from WT and miR-155^−/− mice up an ATP gradient. The experiment was performed 4 times with 3 technical triplicates each. (E) WT and miR-155^−/− DCs were stimulated with 10 ng/mL LPS overnight, and P2rx7 expression on the mRNA level was determined by qRT-PCR (n = 2/group). (F) Expression of P2X7 protein on WT and miR-155^−/− DCs was determined by flow cytometry. Data were pooled from 4 independent experiments.