MiR-155^−/− DCs exhibit broad dysregulation of MAPK signaling. (A) Expression of the 70 most highly up- and downregulated genes in the Kyoto Encyclopedia of Genes and Genomes MAPK pathway in microarray data from WT and miR-155^−/− DCs after stimulation with 50 ng/mL LPS and 5 mM ATP for 15 minutes. Genes contributing to path enrichment were identified and ordered using Gene Set Enrichment Analysis. (B) DCs were stimulated with 50 ng/mL LPS and 5 mM ATP for 15 minutes. Protein expression of phosphorylated ERK (pERK) was determined by Western blot, expressed relative to total ERK (tERK), and normalized to the stimulated WT group. GAPDH was used as a loading control. One representative blot is shown. For the quantification, data were pooled from 3 independent experiments.