MiR-155^−/− DCs display decreased levels of cleaved caspase-1 and IL-1β. (A) A heat map showing known and potential inflammasome component gene expression in a microarray analysis of WT and miR-155^−/− DCs after stimulation with 50 ng/mL LPS and 5 mM ATP for 15 minutes. (B) DCs were stimulated with 50 ng/mL LPS and 5 mM ATP for the indicated time intervals. Protein expression of cleaved caspase-1 (caspase-1 p10) was determined by Western blot and normalized to the stimulated WT group. GAPDH was used as a loading control. One representative blot is shown. For the quantification, data were pooled from 6 independent experiments. (C) On day 7 or 12 of DC culture, the cells were stimulated with 50 ng/mL LPS and 5 mM ATP for the indicated time intervals. IL-1β concentrations were measured in the culture supernatant (n = 3/group). Basal IL-1β production was subtracted.