Modulation of chemokine receptors expression by sHLA-G on PB NK cells. (A-G) Cytofluorimetric analysis of chemokine receptor expression on purified total PB NK cells, gating on CD56^bright or CD56^dim NK cells. (A) Representative staining with anti-CD56 mAb. CC chemokine receptor expression on CD56^bright (B) and CD56^dim (C) NK cells, CXC chemokine receptor expression on CD56^bright (D) and CD56^dim (E) NK cells, and CX₃CR1 expression on CD56^bright (F) and CD56^dim (G) NK cells, either untreated (black bars) or treated with sHLA-G (white bars). Data are expressed as MRFI. Means of 5 different experiments ± SD are shown. Asterisks indicate significant differences. The insets in panels B, D, E, and G show representative stainings of NK cells, ctr, or treated with sHLA-G, with anti-CCR2, anti-CXCR3, and anti-CX₃CR1 mAbs, respectively. Black profiles indicate staining with specific mAbs, whereas gray profiles indicate staining with irrelevant isotype-matched mAb. (H-I) Cytofluorimetric analysis of chemokine receptor expression on purified PB CD56^bright NK cells. (H) Representative staining with anti-CD56 mAb. (I) CCR2 and CXCR3 expression on CD56^bright NK cells untreated (black bars) or treated with sHLA-G (white bars). Data are expressed as MRFI. Means of 5 different experiments ± SD are shown. Asterisks indicate significant differences. The inset shows representative stainings of CD56^bright NK cells, ctr, or treated with sHLA-G, with anti-CCR2 and anti-CXCR3 mAbs, respectively. Black profiles indicate staining with specific mAbs, whereas gray profiles indicate staining with irrelevant isotype-matched mAb.