Modulation of chemokine receptor expression by sHLA-G on NK cells from tonsil. Cytofluorimetric analysis of chemokine receptor expression on purified total NK cells from tonsil, gating on CD56^bright or CD56^dim NK cells on the basis of CD56 expression. (A) Representative staining with anti-CD56 mAb. The expression of chemokine receptors was evaluated on CD56^bright (B) and CD56^dim (C) NK cells, either untreated (black bars) or treated with sHLA-G (white bars). Data are expressed as MRFI. Means of 5 different experiments ± SD are shown. Asterisks indicate significant differences. The insets in panels B and C show representative stainings of NK cells, ctr, or treated with sHLA-G, with anti-CXCR3 and anti-CXCR5 mAbs. Black profiles indicate staining with specific mAbs, whereas gray profiles indicate staining with irrelevant isotype-matched mAb.