Expression of activating and inhibitory receptors on sHLA-G–treated NK cells. Cytofluorimetric analysis of the expression of activatory and inhibitory receptors on purified total NK cells, gating on CD56^bright or CD56^dim NK cells on the basis of CD56 expression. Expression of NK-cell receptors was evaluated on PB CD56^bright (A) and CD56^dim NK cells (B) or tonsil CD56^bright (C) and CD56^dim NK cells (D), either untreated (black bars) or treated with sHLA-G (white bars). Data are expressed as MRFI. Means of 5 different experiments ± SD are shown. Asterisks indicate significant differences. The insets in panels A through D show representative stainings of NK cells, ctr, or treated with sHLA-G, with anti-NKG2A and anti-CD94 mAbs, respectively. Black profiles indicate staining with specific mAbs, whereas gray profiles indicate staining with irrelevant isotype-matched mAb.