Modulation of cytokine and chemokine secretion and signal transduction by sHLA-G. Cytokine and chemokine secretion was assessed on supernatants from purified PB CD56^dim and CD56^bright NK cells by antibody array. Means of 3 different experiments ± SD for modulated cytokines and chemokines are shown in panel A (CD56^dim NK cells) and panel B (CD56^bright NK cells). Black bars indicate untreated NK cells, whereas white bars indicate NK cells treated with sHLA-G. Data are expressed as relative density (density of specific spot/ mean of density of housekeeping proteins spot). Asterisks indicate significant differences. Analysis of signal transduction was performed on total PB NK cells either untreated (black bars) or treated with sHLA-G (white bars), gating on CD56^bright (C) and CD56^dim (D) NK cells. Results are expressed as MRFI. Means of 3 different experiments ± SD are shown. Asterisks indicate significant differences.