Figure 4
Plg-RKT regulates Matrigel invasion by monocytes. U937 cells (A) or human peripheral blood monocytes (B; 4 × 105) were placed in the upper chambers of Transwells coated with Matrigel in the presence of plasminogen (200nM) and uPA (50nM) and either anti–Plg-RKT Fab 7H1 (140nM) or isotype control Fab (140nM). Matrigel invasion in response to MCP-1 was quantified as described in “Invasion and chemotactic migration.” Data represent the mean of triplicates ± SEM of total invasion. **P < .001 and *P < .05 compared with isotype control.
