Plg-RKT regulates monocyte chemotactic migration. (A) U937 cells (4 × 105) were placed in the upper chambers of Transwells in the absence (open bars) or presence of MCP-1 alone (hatched bars) or MCP-1 and plasminogen (200nM; closed bars), and cell migration was quantified as described in “Invasion and chemotactic migration.” *P < .05 compared with cells treated with MCP-1 but without the addition of plasminogen. (B) U937 cells were pretreated with EACA (200mM), aprotinin (2.5μM), or amiloride (200μM), and cell migration in the presence of plasminogen was quantified. **P < .001 and *P < .05 compared with cells with plasminogen alone. (C) Cells were preincubated with either mAb 7H1 (■) or isotype control (□) at the indicated concentrations for 30 minutes, and cell migration in the presence of plasminogen was quantified. *P < .05 compared with isotype control mAb. (D) Human peripheral blood monocytes were preincubated with either 7H1 mAb (140nM; ■) or isotype control mAb (140nM; □) for 30 minutes, and cell migration in the presence of plasminogen was quantified. **P < .01 compared with isotype control mAb. Data represent the mean of triplicates ± SEM of total migration.
