American Society of Hematology

Figure 3

$Figure 3. FACS-based separation of cells derived from a second combination of 2 donors by the use of HLA mAbs and verification by qPCR analysis. (A) Flow cytometric analysis. mAbs DK7C11 (anti-HLA-B12) and BVK1F9 (anti-HLA-A8) were used for cell sorting. Graph denotes 10% of the cells analyzed by the FACS in the 0.05% cell mixture. (B) Presort samples and sorted samples were processed for qPCR analysis of the reference gene HCK, the chimeric DNA markers DYZ1, HLA-A*24, HLA-B*51 and HLA-DRB1*10, and the background DNA markers HLA-A*01 and HLA-B*08. Dotted bars, presort samples; shaded bars, positive fractions after sorting; open bars, negative fractions after sorting.$

FACS-based separation of cells derived from a second combination of 2 donors by the use of HLA mAbs and verification by qPCR analysis. (A) Flow cytometric analysis. mAbs DK7C11 (anti-HLA-B12) and BVK1F9 (anti-HLA-A8) were used for cell sorting. Graph denotes 10% of the cells analyzed by the FACS in the 0.05% cell mixture. (B) Presort samples and sorted samples were processed for qPCR analysis of the reference gene HCK, the chimeric DNA markers DYZ1, HLA-A*24, HLA-B*51 and HLA-DRB1*10, and the background DNA markers HLA-A*01 and HLA-B*08. Dotted bars, presort samples; shaded bars, positive fractions after sorting; open bars, negative fractions after sorting.

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