Multimer size of VWF secreted by nontransduced, FVIII-YFP-, and FVIII-YFP Y1680F-transduced BOECs. Plot profiles of VWF multimer patterns of phorbol 12-myristate 13-acetate-induced releasate (A) and lysate of unstimulated cells (B) from nontransduced (lane 1), normal FVIII-YFP (lane 2), and FVIII-YFP Y1680F (lane 3) transduced BOECs. Multimers were analyzed by 2% agarose gel electrophoresis followed by Western blot analysis using rabbit polyclonal anti–human VWF antibody. Plot profiles of blots were generated with ImageJ Software Version 1.44 (National Institutes of Health; www.rsb.info.nih.gov/ij, 1997-2009).