Figure 4
VWF strings released from FVIII-containing WPBs show reduced platelet binding properties. Two images of nontransduced (A) and FVIII-GFP-transduced (B) BOECs stimulated with 100μM histamine at 2.5 dyne/cm2 for 10 minutes and subsequently perfused with washed human platelets for 5 minutes. Platelet binding to VWF strings originating from nontransduced BOECs is more pronounced than platelet binding to strings released from FVIII-expressing BOECs. Scale bars represent 100 μm. Arrowheads indicate platelets bound to VWF strings released by FVIII-GFP-transduced BOECs. Images were taken on a confocal laser scanning microscopy using a Zeiss LSM510 equipped with Plan NeoFluar 40×/1.3 oil objective (Carl Zeiss) at 37°C.
