Figure 6
Figure 6. FVIII Y1680F variant binds to VWF strings when expressed by BOECs or exogenously added. (A) Nontransduced, FVIII-YFP-, and FVIII-YFP Y1680F-transduced BOECs were stimulated with 100μM histamine at 2.5 dyne/cm2, fixed and stained for VWF (red) and FVIII (green). (B) Nontransduced BOECs were stimulated with 100μM histamine at 2.5 dyne/cm2 followed by perfusion with 5 U/mL purified FVIII-YFP or 5 U/mL FVIII-YFP Y1680F. Cells were fixed and stained for VWF (red) and FVIII (green). Scale bars represent 20 μm.

FVIII Y1680F variant binds to VWF strings when expressed by BOECs or exogenously added. (A) Nontransduced, FVIII-YFP-, and FVIII-YFP Y1680F-transduced BOECs were stimulated with 100μM histamine at 2.5 dyne/cm2, fixed and stained for VWF (red) and FVIII (green). (B) Nontransduced BOECs were stimulated with 100μM histamine at 2.5 dyne/cm2 followed by perfusion with 5 U/mL purified FVIII-YFP or 5 U/mL FVIII-YFP Y1680F. Cells were fixed and stained for VWF (red) and FVIII (green). Scale bars represent 20 μm.

Close Modal

or Create an Account

Close Modal
Close Modal