HLA-A*24:02-restricted and WT1 peptide-specific cytotoxicity mediated by WT1-siTCR–transduced CTLs after repeated stimulation with WT1 peptide. (A) Cytotoxicity of WT1-siTCR-transduced CD8⁺ T cells that had been cultured continuously for > 2 months against WT1 peptide-loaded or -unloaded C1R-A*24:02 cells and C1R cells was examined by 5-hour ⁵¹Cr-release assays. (B) Cytotoxicity of WT1-siTCR–transduced CD8⁺ T cells that had been cultured continuously for > 2 months against HLA-A*24:02-positive and HLA-A*24:02-negative leukemia cell lines was examined by 5-hour ⁵¹Cr-release assays. HLA class I-restriction of cytotoxicity mediated by WT1-siTCR–transduced CTLs against MEG01 and KAZZ cell lines was examined by 5-hour ⁵¹Cr-release assays at an E/T ratio of 5:1 in the presence or absence of anti-HLA class I MoAb or anti-HLA-DR MoAb.