Antileukemia effect of adoptively transferred WT1-siTCR–transduced CTLs in a xenograft mouse model. WT1-siTCR–transduced CTLs and non–gene-modified human CD8⁺ T cells (control CTLs) were prepared from peripheral blood CD8⁺ T cells. NOG mice were inoculated with K562-A*24:02 cells preincubated with WT1-siTCR–transduced CTLs or control CTLs with or without additional cell therapy. (○) represents the growth of leukemia cells in 2 control mice inoculated with K562-A*24:02 cells preincubated with control CTLs and into which control CTLs were transferred weekly; gray circles, leukemia cell growth in 2 mice inoculated with K562-A*24:02 cells preincubated with WT1-siTCR–transduced CTLs without additional transfer of CTLs; and (●), the growth of leukemia cells in 2 mice inoculated with K562-A*24:02 cells preincubated with WT1-siTCR–transduced CTLs, and into which WT1-siTCR–transduced CTLs were additionally transferred weekly. The time points (in days) after transplantation of K562-A*24:02 cells when the mice died are indicated.