Lack of an inhibitory effect of WT1-siTCR–transduced CTLs on human hematopoiesis in a humanized mouse model. HLA-A*24:02-positive hCB-CD34⁺ cells preincubated with autologous WT1-siTCR–transduced CTLs or non–gene-modified autologous CTLs (as a negative control) were transplanted into NOG mice. Three months later, the mice were killed and examined for engraftment and differentiation of human hematopoietic progenitor cells in the spleen and bone marrow. (A) Representative data for 3 experiments. (B) Summary of long-term hematopoiesis of engrafted human hematopoietic progenitor cells in the bone marrow and spleen of mice transplanted with hCB-CD34⁺ cells that had been preincubated with control CTLs or WT1-siTCR–transduced CTLs.