Up-regulation of costimulatory molecules by IL-36β and LPS in IL-36R ^+/+ BMDCs. BMDCs from WT (left panels) and IL-36R^−/− (right panels) mice were either not stimulated (thin gray line) or stimulated with 100 ng/mL of IL-36Ra, IL-36β, or LPS (thick black line) for 24 hours. Cells were then stained for CD80, CD86, or MHC II (I-A^b) before analysis by flow cytometry. Histograms were gated on CD11c⁺ cells. Data are representative of 1 experiment of 2.