PTEN deficiency enhances BMMC proliferation and differentiation. (A) Bone marrow cells from WT (red) or PTEN-null mice (blue) were stained with CFSE and analyzed for cell division on day 7, day 13, and day 23 of culture. Fluorescence intensity was measured by flow cytometry. One representative of 4 experiments is shown. (B) Percentage of FcϵRI and Kit double-positive cells in bone marrow cell cultures of the indicated genotype over time. Cells (1 × 105 cells) were labeled with PE-MAR-1 (anti-FcϵRI) and APC–anti-Kit. Mean fluorescence intensity was determined by flow cytometry. (C) BMMCs (1 × 104) of the indicated genotypes were incubated with 3H-thymidine (1 mCi) and IgE, SCF, or IgE plus SCF. After 12 hours, the amount of incorporated thymidine was determined. (B-C) A minimum of 4 experiments were conducted.
