p13 translocates to the nucleus when coexpressed with Tax. HeLa cells were transfected with p13-HA (2μg) or Tax (0.5μg) and fixed 24 hours after transfection (A-D). DNA concentrations were adjusted using pMH plasmid. Subcellular localization of p13 was examined by immunofluorescence with anti-HA and anti-CoxIV (A) and anti-HA and anti-SC35 (B). Tax localization with anti-Tax and anti-SC35 (C) and anti-Tax and anti-CoxIV (D) antibodies is shown. (E-G) HeLa cells transfected with the p13-HA (2μg) and Tax (0.5μg) constructs and fixed 24 hours after transfection. Subcellular localization of Tax and p13 was examined by immunofluorescence with anti-HA and anti-Tax antibodies (E). Subcellular localization of p13 was analyzed with anti-HA and anti-SC35 (F) and with anti-HA and anti-sp100 and (G). An overlay of these images is shown on the right.