American Society of Hematology

Figure 3

$Figure 3. Intestinal epithelium restricted expression of CX3CL1. (A) Flow cytometric analysis of intestinal epithelial cell fraction obtained from WT mice and CX3CL1cherry mice. Cells are gated according to scatter (left). Middle dot plot: WT cells. Right plot: CX3CL1cherry cells. Note the presence of cherryint and cherryhigh cells in the CD45− epithelial cell fraction. (B) Two-photon imaging of CX3CL1cherry:CX3CR1gfp cecum. (C) Confocal imaging of terminal ileum of CX3CL1cherry:CX3CR1gfp mouse. (D) Confocal imaging of colon of CX3CL1cherry:CX3CR1gfp mouse. (E) Ileal section of CX3CL1cherry:CX3CR1gfp mouse shown under fluorescent microscope and after periodic acid-Schiff staining. Note that CX3CL1-positive large granular goblet cells. Red represents CX3CL1/cherry; and green, CX3CR1/GFP.$

Intestinal epithelium restricted expression of CX₃CL1. (A) Flow cytometric analysis of intestinal epithelial cell fraction obtained from WT mice and CX₃CL1^cherry mice. Cells are gated according to scatter (left). Middle dot plot: WT cells. Right plot: CX₃CL1^cherry cells. Note the presence of cherry^int and cherry^high cells in the CD45⁻ epithelial cell fraction. (B) Two-photon imaging of CX₃CL1^cherry:CX₃CR1^gfp cecum. (C) Confocal imaging of terminal ileum of CX₃CL1^cherry:CX₃CR1^gfp mouse. (D) Confocal imaging of colon of CX₃CL1^cherry:CX₃CR1^gfp mouse. (E) Ileal section of CX₃CL1^cherry:CX₃CR1^gfp mouse shown under fluorescent microscope and after periodic acid-Schiff staining. Note that CX₃CL1-positive large granular goblet cells. Red represents CX₃CL1/cherry; and green, CX₃CR1/GFP.

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