Toso activates survival signaling pathways. (A) Toso activates Erk signaling pathways in response to CD95 stimulation. Jurkat cells (top panel) or BJAB cells (bottom panel) stably expressing Toso or respective wt control cells were stimulated with 100 ng/mL CD95L for the indicated times. Cell lysates were analyzed for Erk1/2 phosphorylation (top) or total Erk1/2 (bottom) expression. Cells were also treated with PMA as a positive control. Data are representative of 3 experiments. Statistical analysis: Student t test (**P < .01). (B) Toso activates NF-κB in response to TNFα stimulation. Toso knock-down Jurkat cells or wt Jurkat cells were stimulated with 10 ng/mL TNFα for 20 minutes. NF-κB binding activity was analyzed by electrophoretic mobility shift assay. Data are representative of 2 experiments.