Increased macrophage recruitment in LPS-treated ID mice. (A) Absolute number of leukocytes (CD45⁺) analyzed in total liver of IR and ID mice under basal condition or after LPS injection (4 mice for each group). Frequency (left panel) and absolute number (right panel) of inflammatory macrophages (CD45+CD11b+F480+), isolated as described in “Tissue digestion and flow cytometry,” were analyzed in liver (B) and spleen (C) of IR and ID mice treated with or without LPS (4 mice for each group) 6 hours before sacrifice. Error bars indicate SE; *P < .05.