Figure 1
c-Cbl activation downstream of αIIbβ3. Human platelets (3 × 108 platelets/mL) were plated over fibrinogen (100 μg/mL) coated plates for the indicated time points. Reaction was stopped with cold lysis buffer. Lysates were examined for c-Cbl phosphorylation using phosphospecific antibodies for residues Y700, Y731 and Y774 as indicated. Equal protein loading was detected with anti–c-Cbl antibody. Cells were also treated with convulxin (Cvx) to ascertain tyrosine phosphorylation of Cbl (positive control). All blots are representative of 3 experiments.
