Role of SFKs over c-Cbl phosphorylation. (A) To determine the role of SFKs over c-Cbl phosphorylation downstream of αIIbβ3, platelets were incubated for 10 minutes with 10μM of Pan SFKs inhibitor PP2 or its negative control PP3 and then plated over fibrinogen coated plates for the indicated time points. Lysates were analyzed as described on legend from Figure 1. (B) Densitometry analysis of 5 experiments. Bars represent mean ± SEM of the ratio of phosphorylated protein over total protein. Student t test performed (P < .05, no treatment vs PP2).