Functional effect of c-Cbl tyrosine phosphorylation on outside-in signaling-mediated events in human platelets. (A) Human platelets (1 × 10⁶ platelets/mL) were treated with PP2 (10μM) or OXSI-2 (2μM) for 10 minutes before be plated on fibrinogen-coated cover slips for the indicated time point. After 3 times washing with PBS, adherent cells were fixed with 3.7% paraformaldehyde and analyzed with DIC imaging. (B) Clot retraction analysis of human platelets treated with PP2 and OXSI-2.