Role of c-Cbl Y731 on platelet-spreading. (A) Platelets from WT, c-Cbl KO, or c-Cbl^YF/YF were treated with apyrase (0.2 unit/mL) and indomethacin (10μM) and allowed to spread over a fibrinogen-coated cover slip for the indicated time point. After fixation with 3.7% paraformaldehyde, platelets were permeabilized with 0.1% Triton X-100 and stained with Rhodamin-palloidin (red). Platelet morphology was analyzed using confocal microscopy. (B) Surface area quantitation of at least 20 platelets. Graph represents mean ± SEM of platelet area. Student t test performed (P < .05, WT vs c-Cbl KO and WT vs c-Cbl^YF/YF). Representative of 3 different experiments.