DAG1 and NRXN1α colocalize near osteoblasts in muBM. Low (20×) and higher (40×) power microphotographs of serial sections mounted using Permount mounting medium and stained with H&E or anti–α-DAG1, anti-NRXN1α, rabbit IgG isotype Abs. DAG1 and NRXN1α colocalized to active osteoblasts (marked with an arrow). Immunoreactivity was also observed along the periphery of megakaryocytes and erythrocytes for both DAG1 and NRXN1α. Note the lack of immunoreactivity in osteocytes and stromal cells. Micrographs were taken on a Leica DM3000 microscope with PLAN objective ×20 (NA 0.4) or a PLAN objective ×40 (NA 0.65) at room temperature. Images were captured using Leica Application Suite v3.6.0 from Leica Microsystems.