Kinetics of Mucorales-specific T-cell responses by IFN-γ, IL-10, and IL-4 ELISpot assay in the 3 patients with invasive mucormycosis. (A) Patient 1. (B) Patient 2. (C) Patient 3. Yellow columns represent the number of Mucorales-specific T cells producing IL-10; blue columns, the number of Mucorales-specific T cells producing IFN-γ; red columns, the number of Mucorales-specific T cells producing IL-4; and dark gray background, T-cell responses in wells with phytohemagglutinin (PHA). Vertical axis shows the number of spot-forming cells (SFCs) per million peripheral blood mononuclear cells (PBMCs); horizontal axis indicates the time (in days) from the beginning of induction chemotherapy.