Schematic of the CMV-λ6 construct which is transmitted in the transgenic mouse genome. (A) The CMV-λ6 transgenic construct including the full-length immunoglobulin LC containing a leader (L), variable (V), joining (J), and constant (C) domain was expressed using the CMV promoter (pCMV) and capped with the bovine growth hormone polyadenylation sequence (BGH pA). (B) PCR genotyping of a representative litter of 10 pups (lanes 3-12) generated by crossing a transgenic line AL55 female (lane 1) and a wild-type male (lane 2) produced 50% transgene positive offspring as expected. Negative (no DNA, lane 13) and positive (plasmid DNA, lane 14) controls are present. The vertical line indicates that noncontiguous lanes from the same agarose gel image are displayed together.