Expression of the human λ6 LC protein in CMV-λ6 transgenic mice. (A-F) Immunohistochemistry demonstrating human λ LC expression (red-brown staining) in (A) stomach, (B) bladder, (C) pancreas, (D) kidney, and (E) heart. (F) Pancreas stained with human κ LC antibody (negative control) is also shown. Antibodies used were Dako nos. A0193 (λ) and A0191 (κ). (G-I) Immunoblots of human λ LC expression (top panels) in serum (G), pancreas (H), and stomach (I) tissue extracts from transgenic (Tg) and wild-type (WT) littermate mice. β-actin was used as a loading control (H-I bottom panel). The anti–human λ antibody used was Sigma-Aldrich no. L5267. The vertical line indicates that noncontiguous lanes from the same immunoblot image are shown.