LFA-1 blockade results in selective loss of CD4⁺ and CD8⁺ T cells from peripheral LNs. (A) B6 recipients of BALB/c SG were left untreated or treated with anti–LFA-1 or CTLA-4 Ig alone or a combination of anti–LFA-1 plus CTLA-4 Ig, and graft-draining LNs were isolated 9 days after skin graft. Cells were stained with anti-CD4 and anti-CD8 and analyzed by flow cytometry. Data shown are gated on lymphocytes and are representative examples from 3 experiments with 3 animals per group. (B-C) Treatment with anti–LFA-1, alone or in combination with CTLA-4 Ig, decreases the absolute number of CD4⁺ (B) and CD8⁺ (C) T cells within peripheral LNs (P < .0001 compared with untreated controls) and increases the absolute number of CD4⁺ (B) and CD8⁺ (C) T cells in the peripheral blood (P < .05 compared with untreated controls). Summary plots are shown, representing cumulative numbers from 2 independent experiments and 6 mice per group per time point.