LFA-1 blockade results in decreased CD62L expression on CD4⁺ and CD8⁺ T cells in peripheral LNs. B6 recipients of BALB/c SG were left untreated or treated with anti–LFA-1 alone, CTLA-4 Ig alone, or a combination of anti–LFA-1 plus CTLA-4 Ig, and graft-draining LNs and peripheral blood were collected 9 days after skin grafting. Cells were stained anti-CD4, anti-CD8, and anti-CD62L, and analyzed by flow cytometry. (A,C) Open histograms indicate untreated controls; shaded histogram indictes treated animals. Data shown are gated on CD4⁺ or CD8⁺ T cells in draining LNs (A-B) and peripheral blood (C-D) and are representative examples from 4 experiments with 3 animals per group. (B) Summary data of MFI of CD62L expression on CD4⁺ and CD8⁺ T cells isolated from LNs (B) or peripheral blood (D) on day 9 after transplantation. Results showed a statistically significant decrease in CD62L expression on CD4⁺ and CD8⁺ T cells in the LNs both in the presence and absence of CTLA-4 Ig (P < .0001 compared with untreated controls). Combined data from 2 independent experiments with a total of 6 mice per group are shown.