PIM2 expression in human DLBCL samples and cell lines. PIM2 immunohistochemical staining of reactive tonsil: original magnification (A) and 100× (B). IHC staining of DLBCL tissues: negative (C) and positive (D) PIM2 staining. Western blot analysis (E) and qRT-PCR (F) of PIM2, in a panel of DLBCL cell lines characteristics of GC or post-GC. The expression of PIM2 is significantly higher (t test, *P < .02) in cell lines of the ABC-DLBCL subtype. The levels of PIM2 were quantified from the Western blot using ImageJ 1.36b (National Institutes of Health) and normalized against α-tubulin. Data are representative of 2 independent experiments. RQ (2-ΔΔCT) was calculated using Universal Human Reference RNA (Stratagene) as the calibrator. RT-PCR reactions were performed in triplicate.