Inhibition of PIM kinases induces apoptosis and cell-cycle arrest in DLBCL cell lines. (A) Induction of apoptosis after treatment for 24 and 48 hours with ETP-39010 at 10μM. The experiment was carried out in triplicate, and SD from the mean is represented with error bars. Statistical comparison of treated (ETP-39010) and untreated (DMSO) cells is indicated (*P < .05; **P < .001). (B) Cell-cycle analysis of DLBCL cell lines treated with ETP-39010 (green line) or vehicle alone (DMSO; red line) was performed for 24 and 48 hours. Cells were stained with PI and analyzed for cell-cycle distribution by flow cytometry. HBL-1, OCILY-10, and OCILY-3 cells were arrested in G1 phase and underwent time-dependent apoptosis. The experiment was carried out in triplicate, and SD from the mean is represented in parentheses.
