Vegfa expression is reduced in LSK CD34⁻ cells from Vegfa^δ/δ BM but not in more differentiated BM cells.Vegfa expression was measured by Q-RT-PCR in (A) unfractionated BM, (B) c-kit enriched cells, (C) LSK CD34⁺ cells (P = .2072, t test), and (D) LSK CD34⁻ cells (*P = .0426, t test) from Vegfa^δ/δ or WT mice. Vegfa expression was normalized to Actb expression. Bars represent mean ± SD. mRNA levels of (E) Vegfa (*P = .0088, t test), (F) Pgk1 (P = .8924, t test), and (G) Hk2 (P = .5203, t test) were measured in c-kit enriched cells from Vegfa^δ/δ or WT mice previously incubated in normoxic or hypoxic conditions for 12 hours. Data in panels E,F, and G are presented as fold increase in gene expression compared with normoxia. Bars represent mean ± SD. Results shown are data combined from 3 independent experiments. N = normoxia, H = hypoxia.