Reconstitution capacity of Ezh2-deficient BM cells is almost intact. (A) Schema of competitive reconstitution assays to evaluate Ezh2-deficient BM cells. BM cells from 8-week-old Cre-ERT and Cre-ERT;Ezh2^fl/fl mice were transplanted into lethally irradiated recipient mice with the same number of competitor BM cells (1 × 10⁶ cells). At 8 weeks after transplantation, recipient mice were injected with tamoxifen for 5 consecutive days; and after a further 12 weeks, secondary transplantation was performed. (B) Donor chimerism in PB at 12 weeks after primary and secondary transplantations. Donor chimerism in PB at the point just before tamoxifen injection was arbitrarily set to 100%. Data are mean ± SD (n = 6): ■ represents wild-type BM cells; and , Ezh2^Δ/Δ BM cells. (C) Donor chimerism in BM KSL cells at 12 weeks after primary transplantation. Data are mean ± SD (n = 4).