Human miR-27a* is a negative regulator of NK-cell cytotoxicity. (A top) Sorting of the GFP-positive NK-92 cells expressing pre-miR-27a*/miR-27a. (Bottom) Real-time qPCR for miRNAs. (B) NK-92 cells expressing pre-miR-27a*/miR-27a show decreased NK-cell cytotoxicity. (Top) IB analysis. (Middle) Real-time qPCR for mRNAs. (Bottom) Cr release assay. Data are representative of 3 independent experiments (mean ± SEM of triplicates; Student t test, **P < .01 and ***P < .001 vs LV_Ctrl. (C-E) miR-27a* negatively regulates NK-cell cytotoxicity in the primary NK cells. Primary human NK cells transfected with Ctrl-miR or miR-27a* (C) or miR-27a* inhibitor (D) were subjected to IB analysis; Cr release assay at ET5, ET1.5, or both; and ELISA for TNF-α (red) and IFN-γ (blue, E). Data are representative of 2 independent experiments (mean ± SEM of triplicates; Student t test, ***P < .001 vs Ctrl_miR).