SFRP1 mRNA levels in patients with AML.SFRP1 mRNA levels in 41 diagnostic BM samples from patients with AML (27 children and 14 adults) were measured by real-time RT-PCR and were normalized with GAPDH. The relative quantity of the mRNAs was determined in triplicates with the use of serial dilutions of cDNA from A549 cells as calibration curves. Results are expressed in log₁₀ scale. Patients were divided into 2 groups according to their t(8;21) (A) or SFRP1 methylation (B) status. Each triangle represents one patient, and the number of patients in each group is shown. Horizontal lines indicate the mean SFRP1/GAPDH ratio.